Continuous-flow In-droplet Magnetic Particle Separation in a Droplet-based Microfluidic Platform

ABSTRACT We demonstrate a droplet-based microfluidic device in which in-droplet magnetic bead separation is performed in continuous-flow. Two droplets containing different solution for the magnetic bead separation are generated in pair-wise manner. Sequential fusion of droplets occurs by a geometrical structure. The magnetic beads are deflected by externally applied magnetic force from one side of droplet to the other. The droplets are split into two daughter droplets, separating magnetic beads at a T-junction. In the experiments we analyze working zone for the separation, adjusting magnet position and total flow rate and measure separation error rate for the optimization of the device.